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Heat-labile UDG

Posted on January 15, 2022 By Valerie Holmes

Description

Uracil-DNA Glycosylase cleaves uracil from dU-containing DNA by breaking the N-glycosidic bond between the uracil base and the sugar backbone. This cleavage generates alkali-sensitive apyrimidinic sites that DNA polymerase blocks from replication or prevents from becoming a hybridization site. Single-stranded and double-stranded dU-containing DNA are substrates for uracil-DNA glycosylase, whereas normal dT-containing RNA and DNA are not.

Uracil-DNA glycosylase can also be used to increase the cloning efficiency of PCR products that have incorporated dU-containing primers, as well as to increase the efficiency of site-directed mutagenesis. Uracil-DNA Glycosylase derived from Gadus morhua has all the attributes of the enzyme derived from E. coli with the added benefit of being heat-labile. It is completely and irreversibly inactivated after 10 minutes of incubation at 50°C.

Specifications

  • Quantity: 100U
  • Kind of product: Uracil DNA Glycosylase
  • Enzyme: glycosylase
  • Compatible buffer: storage buffer

Content and storage

Shipped on dry ice. Store at -20°C.

Purity:

The enzyme is chromatographically purified and analyzed for contaminating endonucleases and exonucleases.

Storage buffer:

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

Test conditions:

The reaction mixture contains 70 mM Tris-HCl, pH 8.0, 10 mM NaCl, 1 mM EDTA, 100 µg/mL BSA, 3 H-dUTP-labeled DNA, and uracil-DNA glycosylase. Incubation is at 37°C for 1 hour.

Unit definition:

One unit is the amount of enzyme required to release 1 nmol of uracil from dU-containing DNA in one hour at 37°C.

Concentration: 1 unit/µL

Applications:

  • Study of DNA repair and mutation detection.
  • Increase the cloning efficiency of PCR products.
  • Increase the efficiency of site-directed mutagenesis.
  • Study of protein-DNA interactions.

Fountain:

Recombinant Gadus morhua (cod liver)

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Recent Posts

  • Mycoplasma pneumoniae Recombinants
  • Canis lupus familiaris Recombinants
  • Cricetulus griseus Recombinants
  • Heat-labile UDG
  • Immunemed HANTA RAPID
July 2022
M T W T F S S
 123
45678910
11121314151617
18192021222324
25262728293031
« Jan    

Categories

  • Antibodies
  • Blog
  • Cultrex
  • Default
  • Dot
  • EIA
  • electrophoresis
  • Exosomes
  • Gels
  • Goat
  • HRP
  • Isotypes
  • NATtrol
  • Particles
  • Rabbit
  • Species

Recent Posts

  • Mycoplasma pneumoniae Recombinants
  • Canis lupus familiaris Recombinants
  • Cricetulus griseus Recombinants
  • Heat-labile UDG
  • Immunemed HANTA RAPID

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